SYP, MRX96, MRXsynaptophysin, MRXSYP, Major Synaptic Vesicle Protein P38
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse Synaptophysin
Isotype
IgG
Purity
Affinity purification
Subcellular location
Synaptic vesicle membrane
Predicted MW.
34 kDa
Observed MW.
38 kDa
Uniprot ID
P08247, Q62277, P07825
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1:5000-1:10000
brain, cerebral cortex, cerebellum
IHC/IF
Human, Mouse, Rat
1:1000-1:2000
brain, cerebellum, epencephalon, spinal cord
Background
Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.
Images
Western blot analysis of Synaptophysin (GB11553) at dilution of 1: 5000
Immunohistochemistry of paraffin embedded mouse brain using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded mouse cerebellum using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded mouse spinal cord using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded rat brain using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded rat cerebellum using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded rat spinal cord using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Immunofluorescence of paraffin embedded mouse brain using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Immunofluorescence of paraffin embedded mouse epencephalon using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Immunofluorescence of paraffin embedded mouse spinal cord using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Immunofluorescence of paraffin embedded rat brain using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Immunofluorescence of paraffin embedded rat epencephalon using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .