服務(wù)熱線
400-1016-218
聯(lián)系方式
- 手機(jī)：400-1016-218
- Q Q：355185756
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室
Anti -EGFP Rabbit pAb
價(jià)格：
在線咨詢(xún)
貨號(hào)：
DRF10129
產(chǎn)品規(guī)格：
100 μL
Applications：
WB | IF
Species：
--
購(gòu)買(mǎi)數(shù)量：
產(chǎn)品詳情
操作說(shuō)明
注意事項(xiàng)
Product Information
|
Description |
EGFP rabbit polyclonal |
|
Protein full name |
Enhanced GFP |
|
Synonyms |
Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
|
Immunogen |
Recombinant protein corresponding to EGFP( enhanced green fluorescent protein) |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
27 kDa |
|
Observed MW. |
29 kDa |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
/ |
1: 500-1: 1000 |
cells/ tissues with eGFP |
|
IF |
/ |
1: 500-1: 1000 |
293T |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
Images
|
Western blot analysis of eGFP (GB11602) at dilution of 1: 500 Lane 1: 293T cell lysate Lane 2: 293T with eGFP cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 with eGFP cell lysate |
|
Immunofluorescence analysis of human 293T cells using eGFP (GB11602) at dilution of 1: 1000 A: DAPI nuclear stain ( blue ) B: eGFP protein ( green ) C: Anti-eGFP ( red ) D: Merged |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
凡合肥萬(wàn)物生物科技有限公司銷(xiāo)售的產(chǎn)品均有嚴(yán)格的質(zhì)量保證。如發(fā)現(xiàn)產(chǎn)品存在包裝規(guī)格疑問(wèn)，請(qǐng)于收貨之日起七日內(nèi)向我公司總部或當(dāng)?shù)剞k事處提出，并請(qǐng)保證該產(chǎn)品，以備本公司進(jìn)行核對(duì)檢驗(yàn)。如果發(fā)現(xiàn)有質(zhì)量問(wèn)題，請(qǐng)于收貨后一月內(nèi)保留產(chǎn)品50%以上的量，以便本公司回收產(chǎn)品進(jìn)行質(zhì)檢，確認(rèn)產(chǎn)品質(zhì)量。如逾期，則視為已經(jīng)對(duì)本公司產(chǎn)品進(jìn)行驗(yàn)收。 聲明：如發(fā)生產(chǎn)品索賠事宜，本公司僅在產(chǎn)品價(jià)格范圍內(nèi)酌情賠償，恕不接受超出產(chǎn)品價(jià)格范圍之賠償?？蛻?hù)在使用過(guò)程中有任何技術(shù)問(wèn)題可以撥打技術(shù)售后服務(wù)電話我們隨時(shí)給予解答。
合肥萬(wàn)物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室