KLH conjugated Synthetic peptide corresponding to Mouse NeuN
Isotype
IgG
Purity
Affinity purification
Subcellular location
Nucleus, Cytoplasm
Predicted MW.
41 kDa
Observed MW.
48 kDa
Uniprot ID
Q8BIF2
Applications
Applications
Species
Dilution
Positive Sample
WB
Mouse, Rat
1:500-1:1000
brain, cortex, hippocampus
IHC/IF
Mouse, Rat
1:500-1:1000
brain, hippocampus
Background
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
Images
Western blot analysis of Neun (GB11138) at dilution of 1: 500
Immunohistochemistry of paraffin embedded rat brain using neun (GB11138) at dilution of 1: 1000 (200x lens)
Immunohistochemistry of paraffin embedded mouse brain using neun (GB11138) at dilution of 1: 500 (300x lens)
Immunofluorescence of paraffin embedded rat hippocampus using neun (GB11138) at dilution of 1: 500 (200x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .