MKI67, marker of proliferation Ki-67, KIA, MIB-, MIB-1, PPP1R105
Immunogen
Recombinant protein corresponding to Mouse Ki67
Isotype
IgG
Subcellular location
Nucleus,nucleolus
Purity
Affinity purification
Predicted MW.
359KDa
Observed MW.
359KDa
Uniprot ID
E9PVX6
Applications
Applications
Species
Dilution
Positive Tissue
WB
Human, Mouse
1:300-1:600
liver, stomach, colon
Background
Required
to maintain individual mitotic chromosomes dispersed in the cytoplasm
following nuclear envelope disassembly (PubMed:27362226). Associates
with the surface of the mitotic chromosome, the perichromosomal layer,
and covers a substantial fraction of the chromosome surface
(PubMed:27362226). Prevents chromosomes from collapsing into a single
chromatin mass by forming a steric and electrostatic charge barrier: the
protein has a high net electrical charge and acts as a surfactant,
dispersing chromosomes and enabling independent chromosome motility
(PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and
AT-rich DNA (By similarity).
Images
Western blot analysis of KI67 (GB11030) at dilution of 1: 300
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .