Anti -Histone H2A.X Rabbit pAb

價格：在線咨詢

貨號：

KT012187

產品規(guī)格：

Applications：

WB/IHC/IF

Species：

M/R,M/R,M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產品詳情操作說明注意事項

Product Information

Description	Histone H2A.X rabbit polyclonal
Protein full name	Histone H2AX
Synonyms	H2A histone family, member X, H2A.X, H2A/X, H2AFX,H2AX, Histone H2A.x
Immunogen	KLH conjugated Synthetic peptide corresponding to Human Histone H2A.X
Isotype	IgG
Purity	Affinity purification
Subcellular location	Nucleus
Predicted MW.	15 kDa
Observed MW.	15 kDa
Uniprot ID	P16104, P27661

Applications

applications	species	dilution	Positive tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	testis, kidney, thymus
IHC	Mouse, Rat	1: 1600-1: 3200	testis, kidney
IF	Mouse, Rat	1: 100-1: 500	kidney, testis

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Images

	Western blot analysis of H2AX (GB111560) at dilution of 1: 1000 Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse thymus tissue lysate Lane 7: Rat testis tissue lysate Lane 8: Rat kidney tissue lysate Lane 9: Rat thymus tissue lysate
	Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111560) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111560) at dilution of 1: 2000
	Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111560) at dilution of 1: 300
	Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using H2AX (GB111560) at dilution of 1: 300
	Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111560) at dilution of 1: 300
	Immunofluorescent analysis of paraformaldehyde-fixed rat testis using H2AX (GB111560) at dilution of 1: 300

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

凡合肥萬物生物科技有限公司銷售的產品均有嚴格的質量保證。如發(fā)現(xiàn)產品存在包裝規(guī)格疑問，請于收貨之日起七日內向我公司總部或當?shù)剞k事處提出，并請保證該產品，以備本公司進行核對檢驗。如果發(fā)現(xiàn)有質量問題，請于收貨后一月內保留產品50%以上的量，以便本公司回收產品進行質檢，確認產品質量。如逾期，則視為已經(jīng)對本公司產品進行驗收。聲明：如發(fā)生產品索賠事宜，本公司僅在產品價格范圍內酌情賠償，恕不接受超出產品價格范圍之賠償?？蛻粼谑褂眠^程中有任何技術問題可以撥打技術售后服務電話我們隨時給予解答。

合肥萬物生物科技有限公司

客服熱線：400-1016-218

QQ：355185756

地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室