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Anti -Villin Rabbit pAb

價格: 在線咨詢
貨號:
KT012262
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
購買數(shù)量:
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Product Information



Description

Villin rabbit polyclonal antibody

Protein full name

Villin-1

Synonyms

VIL1, VIL, Villin 1, D2S1471

Immunogen

Recombinant protein corresponding to Human Villin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

93 kDa

Observed MW.

95 kDa

Uniprot ID

P09327, Q62468





Applications



Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

small intestine, colon, kidney

IHC/IF

Human, Mouse, Rat

1: 500-1: 3000

colon, gall bladder, kidney, small intestine





Background


Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.




Images


Western blot analysis of villin  at dilution of 1: 1000

Immunohistochemistry of paraffin embedded human colon using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded mouse kidney using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded rat gall bladder using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded rat small intestine using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunofluorescence of paraffin embedded human colon using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunofluorescence of paraffin embedded mouse kidney using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunofluorescence of paraffin embedded rat gall bladder using villin (GB111209) at dilution of 1: 1500 (400x lens)

Immunofluorescence of paraffin embedded rat small intestine using villin (GB111209) at dilution of 1: 1500 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol
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